Search the database

The search function allows you to collate information on defined mutations in resistance associated genes or analyse your own DNA/protein sequences for resistance mutations.

Select gene

Analyse Mutations

Type in a defined mutation or series of mutations to find published information:
Format: [WT][POS][MUT] or [POS][MUT]
([WT] = AS in wildtype, [POS] = position in protein, [MUT] = AS in mutant))
Example: F46Y M172V

Scan sequence


Using this function you can analyze your own DNA or protein sequence. The CYP51A Gene can be amplified with 3 primer-pairs (i.e. Cyp1-L/Cyp1-R, Cyp2-L/Cyp2-R, Cyp3-L/Cyp3-R (Chen et al. 2005), Cyp1-L and Cyp3-R being located outside the coordinated region. The amplification is carried out as described by (Chen et al. 2005) with an elevated number of cycles : 95°C 5min, 36 cycles 95°C 30sec, 58°C 30sec, 72°C 1min; one cycle 72°C 10min in a PCR approach using commercial ingredients. Sequencing of the 3 products is done with both primers each. This will result in three sequences covering the entire CYP51A gene, which can be pasted in as sequence 1,2 and 3. The algorithm will automatically extract relevant sequence information. Therefore any sequence obtained by other methods, partially or completely covering A. fumigatus CYP51A can also be entered and analysed using our algorithm. Our algorithm does also provide a tool for analyzing sequence repeats in the CYP51A promoter region. For sequencing the promoter region, i.e. the primer-pair A5/A7 (Mellado et al. 2001) can be used.

DNA-Sequence 1 (max 5000 bp)

DNA-Sequence 2 (max 5000 bp)

DNA-Sequence 3 (max 5000 bp)