Questions and Answers

Important Note

FunResDb is a curated database integrating published information on A. fumigatus CYP51A mutations. Although we carefully extract information from published resources we cannot guarantee that all information is correct. For this purpose, users of FunResDb should always check the original publications (links to PubMed are provided by FunResDb). Furthermore, phenotypic resistance can occur through different mechanisms. In some patient cohorts, up to 43% of A. fumigatus strains resistant to azoles have been found to have wild-type CYP51A (Bueid et al., 2010). Therefore, identification of a wild-type CYP51A sequence does not automatically infer susceptibility. If you identify mistakes in FunResDb, we are grateful if you let us know and will try to continuously improve the database.
The German National Reference Center for Invasive Fungal Infections NRZMyk is the national reference laboratory for diagnosis and epidemiological analysis of fungal infections appointed by the Robert Koch – Institute. We are funded by the Robert Koch – Institute from funds provided by the Federal Ministry of Health (1369-240). The NRZMyk is located at the Leibniz Institute for Natural Product Research and Infection Biology – Hans-Knoell-Institute in Jena. We provide a broad range of diagnostic services including molecular diagnostics, susceptibility testing and species identification of human pathogenic fungi. Please find more detailed information on our website ( ) .
All the data has been extracted by careful reading of published literature which investigated azole resistance of clinically relevant strains of A. fumigatus. Published information was extracted from the manuscript following the interpretation of the authors. As methods used for susceptibility testing as well as breakpoints used for data interpretation may differ between published studies, we refer our users to the original publications that can easily be accessed via our database.
A DNA or protein sequence of A. fumigatus CYP51A serves as input for the database. In case of DNA sequence, the exon structure is determined by the algorithm in order translate the coding nucleotides into the corresponding amino acids. Next, the given protein sequence is compared to a wild-type sequence to detect amino acid substitutions and output their positions. The result are mutation identifiers which consist of three parts: wild-typ amino acid, position in the protein sequence, mutated amino acid (e.g. L46T; WT: leucine at position 46; mutated to tyrosine). Mutation identifiers can be searched in the database to retrieve documented A. fumigatus strains which contain those patterns.
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